Unlock the potential of deep imaging with this workshop on Optical Tissue Clearing Techniques, designed to complement 3D lightsheet microscopy. Tissue clearing transforms biological specimens into transparent structures, enabling unprecedented imaging depth without physical sectioning. When combined with lightsheet microscopy, tissue clearing allows researchers to visualize intact 3D biological structures—such as neuronal networks and whole organs—with high precision and minimal photobleaching.

This one-and-a-half-day workshop offers hands-on demonstrations in tissue clearing workflows optimized for light sheet microscopy. Participants will be introduced to key concepts through a combination of introductory lectures and live demonstrations. Topics include sample preparation for various tissue types, mounting strategies for systems such as the Zeiss Lightsheet 7 and Miltenyi UltraMicroscope Blaze, as well as basic data management and processing for large datasets.

With an emphasis on adaptability, attendees will learn how to optimize clearing protocols for their specific samples and fluorescence labels—empowering them to apply these techniques to their own research projects.

The workshop is structured into three focused sessions:

CARL ZEISS – 3D imaging of large, cleared samples using the Zeiss Lightsheet 7
Miltenyi Biotec – UltraMicroscope Blaze for 3D imaging at cellular resolution
Imaging smaller cleared samples with confocal microscopy