Programme:
Coming!
Sessions:
Combining complementary imaging modalities can generate a highly informative, composite view of the sample and allows access to both structural and functional parameters across all relevant scales from the same sample. While the correlation of light with transmission electron microscopy (EM) is well established (generally referred to as CLEM), the frontiers of bioimaging are currently being pushed towards the integration of other modalities into correlative workflows, including both super-resolution and large-scale imaging techniques such as light sheet, X-ray or Volume EM to study intercellular relationships and put molecular information into its overall structural context.
Observing sub-cellular structures at the smallest scale is crucial in biomedical research for deepening our understanding of biological processes. Recent advancements include faster acquisition speeds, tracking single molecule trajectories during imaging, high-throughput approaches, and the development of new probes. This enhanced resolution is achieved through specialized sample preparation techniques, optical engineering, and image analysis. It allows the exploration of nanoscale structures within cells and tissues. This session will discuss novel advancements in super-resolution imaging, including methods that facilitate super-resolution imaging in live cells, such as MINFLUX, MIN-STED, and SR-SIM, rapid single molecule imaging, and Expansion Microscopy will be highlighted.
Temporal imaging is essential to uncover and quantify complex dynamic information in biological systems. In tissues and organs, cells form complex, functional structures and interact dynamically. This session will focus on multi-scale live imaging, from 2D and 3D cell cultures to whole organisms, and will highlight the role of state-of-the-art imaging techniques (e.g., light-sheet and 3-Photon Microscopy) in the unveiling of new biological processes. Among the topics covered by this session are recent advances in organoid imaging, tissue imaging and intravital imaging used to study cell-cell interactions, proliferation and differentiation during development and regeneration
Smart microscopy refers to microscopes that can perform real-time analysis and adjust their imaging parameters on-the-fly based on feedback from the acquired images, without human intervention. These microscopes utilize feedback loops to directly read and process the images, and then feed that information back to optimize the imaging regime.
This session will focus in the latest advancements in imaging technologies that enable real-time observation and analysis of live cells with unprecedented resolution and accuracy. These applications merge state-of-the-art techniques such as super-resolution microscopy, advanced fluorescence labeling, and innovative optical engineering to capture dynamic cellular processes as they unfold. This approach provides deep insights into cellular behaviors, interactions, and functions within their natural environment, significantly enhancing our understanding of complex biological systems at the nanoscale.
Workshops:
In advance of BNMI 2025 Symposium, we are pleased to offer a series of hands-on pre-Symposium workshops on August 18 and 19. These sessions will cover cutting-edge topics, including:
- Correlative Array Tomography
- Smart Microscopy
- Optical Tissue Clearing Techniques for 3D Lightsheet Imaging
These workshops offer a valuable opportunity for participants to gain hands-on experience with advanced imaging techniques before the main symposium. Join us to deepen your technical expertise and expand your research toolkit! The pre-symposium workshops are free for those who register and attend the main event. Due to limited space, we request a brief motivation letter and a short biosketch from each applicant. The pre-symposium workshops are free of charge; however, registration is required. Due to limited availability, we kindly ask each applicant to submit a brief motivation letter and a short biosketch.
Registration link coming soon!